Complex DNA sequence readout mechanisms of the DNMT3B DNA methyltransferase
نویسندگان
چکیده
منابع مشابه
Studies of Mechanisms Controlling Dna Methyltransferase 1 Studies of Mechanisms Controlling Dna Methyltransferase 1
We present a simple, non-radioactive assay for DNA methyltransferase activity and DNA binding. As most proteins are studied as GFP fusions in living cells, we used a GFP binding nanobody coupled to agarose beads (GFP nanotrap) for rapid one-step purification. Immobilized GFP fusion proteins were subsequently incubated with different fluorescently labeled DNA substrates. The absolute amounts and...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2020
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkaa938